Composite

Part:BBa_K4624627:Design

Designed by: Theofilos Terzopoulos   Group: iGEM23_Thessaly   (2023-10-02)


AraC/ParaBAD-Na.SP-laccase-rrnB T1/T7TE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2020
    Illegal BglII site found at 2452
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 2052
    Illegal XhoI site found at 2685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2538
    Illegal NgoMIV site found at 2691
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1419
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1484


Design Notes

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Source

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References